Thiol-Based Probe Linker with Antifouling Properties for Aptasensor Development

Surfaces with antifouling properties are critical for optimizing biosensors to improve the selectivity and specificity of analyte detection in complex biological samples. This work describes four-step synthesis 3-dithiothreitol propanoic acid (DTTCOOH), a new thiol linker that (a) significantly reduces fouling raw human serum samples (b) binds amino receptors via its terminal carboxylic group. DTTCOOH was successfully functionalized on quartz crystal microbalance (QCM) discs used anchor penicillin-binding aptamers. Relative bare coated (11-mercaptoundecanoic (MUA) 1-undecanethiol (UDT)) QCM crystals, DTTCOOH’s improved by approximately 75–86%. Following aptamer/ethanolamine extension, modified layer reduced 95–97% compared (MUA, UDT) crystals. dissipation (QCM-D) monitoring, contact goniometry, cyclic voltammetry techniques were compare surfaces crystals hydrophobic hydrophilic molecules.